MEASUREMENTS OF MUSCLE PHYSIOLOGICAL CROSS-SECTIONAL AREAS OBTAINED BY POINT COUNTING AND METAMORPH

Teresa García-Garrido; Antonio Blázquez-Zaballos; María Rosa Sánchez-González; Lorena Benito-Garzón

doi:10.5566/ias.3760

Authors

Teresa García-Garrido Service de Soins Intermédiaires de Pédiatrie. Hôpital des Enfants.Centre Hospitalier Universitaire Vaudois
Antonio Blázquez-Zaballos Department of Statistics. Statistics and Operational Research. Faculty of Economics and Business.
María Rosa Sánchez-González Department of Human Anatomy and Histology. Faculty of Medicine
Lorena Benito-Garzón Department of Human Anatomy and Histology. Faculty of Medicine

DOI:

https://doi.org/10.5566/ias.3760

Keywords:

statistics, stereology, histomorphometry, muscle histochemistry, metamorph

Abstract

Histochemical studies of striated muscles are the most widely used tool for examining their functional properties. The fibres that make up these muscles can be identified by revealing the enzymes involved in contraction, which makes it possible to determine whether they perform resistance (aerobic) or strength (anaerobic) functions. These functions are analysed by comparing the physiological cross-sectional areas of the muscles, regardless of the number of fibres that constitute them or their individual diameters. Areas are measured using an estimated stereological point counting technique in which points are associated with surfaces. This work proposes an automatic, computerised measurement method using the histomorphometric programme Metamorph and compares the results obtained through both methods.

Author Biographies

Teresa García-Garrido, Service de Soins Intermédiaires de Pédiatrie. Hôpital des Enfants.Centre Hospitalier Universitaire Vaudois

Service de Soins Intermédiaires de Pédiatrie. Hôpital des Enfants.Centre Hospitalier Universitaire Vaudois
Antonio Blázquez-Zaballos, Department of Statistics. Statistics and Operational Research. Faculty of Economics and Business.

Department of Statistics. Statistics and Operational Research. Faculty of Economics and Business.
María Rosa Sánchez-González, Department of Human Anatomy and Histology. Faculty of Medicine

Department of Human Anatomy and Histology. Faculty of Medicine
Lorena Benito-Garzón, Department of Human Anatomy and Histology. Faculty of Medicine

Associate Profesor of Histology. Department of Human Anatomy and Histology. Faculty of Medicine.

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